PHF11 promotes DSB resection, ATR signaling, and HR

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PHF11 promotes DSB resection, ATR signaling, and HR.

Resection of double-strand breaks (DSBs) plays a critical role in their detection and appropriate repair. The 3' ssDNA protrusion formed through resection activates the ATR-dependent DNA damage response (DDR) and is required for DSB repair by homologous recombination (HR). Here we report that PHF11 (plant homeodomain finger 11) encodes a previously unknown DDR factor involved in 5' end resectio...

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Activation of DSB processing requires phosphorylation of CtIP by ATR.

DNA double-strand breaks (DSBs) activate a DNA damage response (DDR) that coordinates checkpoint pathways with DNA repair. ATM and ATR kinases are activated sequentially. Homology-directed repair (HDR) is initiated by resection of DSBs to generate 3' single-stranded DNA overhangs. How resection and HDR are activated during DDR is not known, nor are the roles of ATM and ATR in HDR. Here, we show...

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DNA double-strand breaks (DSBs), i.e. where both strands of the DNA double helix are broken, are among the most toxic type of damage that cells can suffer. They can arise during normal cellular processes or are induced by commonly used anticancer modalities, such as ionising radiation. Unrepaired DSBs can result in cell death, and their miss-repair drives genome rearrangements and the loss of g...

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ژورنال

عنوان ژورنال: Genes & Development

سال: 2017

ISSN: 0890-9369,1549-5477

DOI: 10.1101/gad.291807.116